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We investigated the effects of sensory conditioning and sensory stimulation upon GluR2 mRNA expression in the somatosensory barrel cortex. It was previously found that classical conditioning, where the stimulation of vibrissae was paired with a tail shock, induced plastic changes in the cortical representations of vibrissae activated during the training. One hour and 24 h after training or just vibrissal stimulation without the tail shock, we examined GluR2 mRNA expression changes with in situ hybridization using radioactive [35S] oligoprobes. We found that 1 h after the end of training, the hybridization signal was elevated in layer IV in the cortical representations of the "trained" or stimulated vibrissae, while after 24 h it was observed only in the trained group. The results suggest a change of subunit composition of AMPA receptors which can possibly be a protective mechanism that may counteract the effects of strong excitation. The data provide unique observation of GluR2 mRNA regulation in an intact brain and in a behavioral context.
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Time 10: 30 Abstract # 647 Evaluation of Dorset, Finnsheep, Romanov, Texel, and Montadale breeds of sheep: Reproduction of F1 ewes in spring mating seasons. K. A. Leymaster * , E. Casas, B. A. Freking; USDA-ARS, U.S. Meat Animal Research Center, Clay Center, NE Linear versus threshold model analysis of trainability in a colony of German Shepherd dog guides. J. Cole * 1, E. Leighton2; 1Animal Improvement Programs Laboratory Agricultural Research Service, USDA, Beltsville, MD, 2The Seeing Eye, Inc., Morristown, NJ Developing breeding objectives for Targhee sheep. R. C. Borg * 1, D. R. Notter1, R. W. Kott2, L. A. Kuehn1; 1Department of Animal and Poultry Sciences, Virginia Polytechnic Institute and State University, Blacksburg, 2Department of Animal and Range Sciences, Montana State University, Bozeman Genetic and environmental factors affecting camel reproduction. Suleiman Hermas * ; University of Al-fatah Faculty of Agriculture Dept. of Animal Production, Tripoli-Libya Development of web-based cow-calf decision support software. B.W. Brigham * , D.J. Garrick, R.M. Enns; Colorado State University and naloxone.
Xcel and Mr John Cook, a founder and senior vice president of Xcel, were previously employed by Dura, a company Elan acquired in November 2000. Mr James Fares, a founder and senior vice president of Xcel, was previously employed by Elan. Elan rationalised the product rights and related inventory of Diastat to Xcel on 31 March 2001. Elan subsequently rationalised the product rights and related inventory of Mysoline to Xcel. Both these products fall within Xcel's focus on neurology. Diastat and Mysoline are products used for the treatment of epilepsy. Under the product agreements, Xcel acquired worldwide rights to Diastat and exclusive rights to Mysoline in the United States. Elan received aggregate cash consideration of approximately 0.0 million for Diastat and Mysoline. Elan has a royalty right of between 5% and 20% on net sales of Mysoline by Xcel. After reducing the carrying value of the related intangible assets, Elan recorded net revenue of .0 million and .5 million on the rationalisation of Diastat and Mysoline, respectively, in 2001. On 30 March 2001, Xcel raised net proceeds of .6 million from issuing convertible preferred stock. Elan purchased .0 million of this convertible preferred stock, representing approximately 16% of Xcel's equity on a fully diluted basis. On this date, two venture capital funds and their affiliates purchased 54% of Xcel's equity on a fully diluted basis. On 31 March 2001, Elan provided a loan of .0 million to Xcel. Xcel is obligated to make quarterly payments of interest at an annual rate of 7% on the outstanding balance of the loan through 31 March 2004. Interest and principal payments are then due quarterly through the final maturity date of the loan on 31 March 2008. .1 million of the principal amount of the loan is prepayable, at the option of Xcel, beginning in 2007. Elan also provided a .0 million line of credit to Xcel, available for draw down through June 2002, on broadly similar terms. Xcel has drawn down this amount since 31 December 2001. The loan is secured by the rationalised products and also includes other customary financial and operating covenants to which Xcel is subject. Elan expects that Xcel will generate sufficient revenues from its commercial activities to enable it to repay the loan note. Elan has committed to maintain its fully diluted percentage holding in Xcel through and including the date of Xcel's initial public offering. Mr Erle Mast, an Elan employee, joined Xcel's board of directors in February 2002. Nasarel Nasalide IVAX is engaged in the research, development, manufacturing and marketing of branded and brand equivalent generic ; pharmaceuticals and veterinary products in the United States and international markets. In September 2001, Elan rationalised the product rights and related inventory of Nasarel and Nasalide to IVAX. Elan received cash consideration of approximately 0.0 million for Nasarel and Nasalide and retained a royalty right of between 5% and 10% on net sales of Nasarel and Nasalide by IVAX. After reducing the carrying value of the related intangible assets, Elan recorded net revenue of .6 million on the rationalisation of Nasarel and Nasalide in 2001. Permax Amarin is a specialty pharmaceutical company focused on neurology and pain management. Amarin is a United Kingdom public limited company and is also quoted on The Nasdaq Stock Market's National Market "Nasdaq" ; in the United States. Amarin revenue and net profit for 2001 were .0 million and .4 million, before exceptional charges of .1 million, respectively. As of 31 December 2001, Amarin had 93 employees, including 34 sales and marketing personnel. Mr Thomas Lynch, executive vice chairman of Elan, and Mr John Groom, a director of Elan, serve on Amarin's board of directors. Mr Lynch is non-executive chairman of Amarin. Mr Michael Coffee, a director and chief operating officer of Amarin; Mr Nigel Bell, chief financial officer of Amarin; and Mr Donald Joseph, an executive vice president of Amarin, were previously employed by Elan. In May 2001, Elan and Amarin entered into a distribution and option agreement, whereby Amarin agreed to market and distribute Permax in the United States, and was granted an option to acquire rights to the product from Elan. Permax is used for the treatment of Parkinson's disease and falls within Amarin's focus on neurology. In September 2001, this agreement was amended, whereby Amarin was appointed the sole distributor of Permax in the United States until August 2002. Elan recorded consideration of .0 million under the terms of the amended distribution and option agreement and retained a royalty right of 3.5% on net sales of Permax by Amarin from 1 January 2002 through the date on which Amarin exercises or terminates its option to acquire Permax. In 2001, Elan also recorded a net amount of.
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Bonds with N 2 of His613, O 2 of Asp764, and a water molecule, in addition to interactions with residues Tyr612, His613, Asn661, Ser663, Leu725, Asp764, Leu765, and Phe820 Table 2 ; . A few atoms of rhamnose are located at distance suitable for van der Waals contact with both metal ions and directly interact with some of the metal-binding residues such as Asp764. Although icarisid II and sildenafil occupy the same active site, the detailed interactions of the inhibitors are significantly different Fig. 4 ; . Sildenafil forms two hydrogen bonds with Gln817 and stacks against Phe820. However, Gln817 shows about 90 rotation of its side chain Fig. 4 ; and forms no hydrogen bond with icarisid II. The hydrogen bond between the side chain atoms of O 1 Gln775 and N 2 of Gln817 in the unliganded PDE5 and its complex with IBMX and sildenafil vanishes in the PDE5-icarisid II structure, and the two atoms separate by 4.3 . The conformational change of the side chain Gln817 in the icarisid II structure appears to be a consequence of its unfavorable proximity to the hydrophobic pentenyl and phenyl groups. In addition, the position of oxychromone of icarisid II, which stacks against Phe820, is significantly different from the pyrazolopyrimidinone of sildenafil. In consideration of the 1000-fold difference in binding affinity between sildenafil and icarisid II, the structural data suggest that the hydrogen bond with Gln817 and the stacking against Phe820 are two essential components for high affinity binding of inhibitors. This result is consistent with an established role of Gln817 in providing for high potency of sildenafil, vardenfil, and tadalafil 45 and naltrexone.
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Analyses focused specifically on patient visits where an ICD-9 diagnosis code indicated 1 of the following disorders in the first, second, or third diagnosis field: major depressive disorder; dysthymic disorder; depressive disorders, not otherwise specified; bipolar II disorder; or bipolar disorder, not otherwise specified 296.58, 296.82, 296.89, or 311 ; . Visits for depression were further grouped by medical specialty and psychotropic drug class. Statistical Methods One of the primary aims of the NAMCS is to provide national estimates of the volume and content of office-based care. Because of the complex patient visit sampling design, the NCHS weights each visit to mirror the US population. The US Bureau of the Census population estimate for July 1 of the survey year is used to compute the annual visit prevalence. The percentages reported in the current report are based on the weighted estimates. Estimates for the 1993 and 1994 period represent the annualized mean of the 2 survey years. The NCHS provides formulae for the 1985 and 1993 and 1994 NAMCS reports to calculate SEs of the survey estimates in this complex sample.11-13 These formulae were used to compute 99% confidence intervals around the survey estimate and to accommodate for multiple comparisons. RESULTS Psychotropic Medication Visits As depicted in Table 2, between the 2 study periods, overall visits to office527.
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Comparisons of different FSIGT tests Glucose and insulin values obtained during the FSIGTs were used to calculate SI, which is inversely related to insulin resistance, and fractional glucose utilization independent on insulin SG ; 23, 24 ; . Analyses were performed by the minimal model method, using a computerized algorithm MINMOD ; 23, 24 ; . SI was expressed in units1024 minmU ml, and SG in units1024 min. Furthermore, AIRg incremental insulin above baseline at the different time points between 2 and 10 min of FSIGT number of time points considered ; , the disposition index AIRg SI Y basal insulin effectiveness BIE; SIfasting insulin ; , and glucose effectiveness at zero insulin GEZI; SG2BIE ; were also calculated 28 ; . The same calculations performed on the FSIGT33 were repeated by progressively removing some time points, and thus obtaining the FSIGT22 215, 210, 25 and naratriptan!
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VERTICAL SITE MOTIONS IN THE LOWER RHINE BASIN B. Grres o Geodetic Institute of the University of Bonn, Nussallee 17, D-53115 Bonn, Germany. goerres uni-bonn The Lower Rhenish Embayment as a part of the West European Rift system is known both for its present-day seismo-tectonic activity and its extensive brown coal minig. Since parts of the region are strongly influenced by ground water withdrawal associated with the open-pit mining activities, significant subsidence of up to 2.5 cm a can be observed. Vertical and horizontal motions due to recent tectonics are much smaller by at least an order of magnitude. In order to study the complex crustal processes influenced by fluid motions, especially the large groundwater level changes, geodetic and geophysical data are collected, including horizontal and vertical site motions by GPS, repeated precise levellings and precise gravity measurements. The GPS results confirm that the precision for the height component has improved to the level of 3 mm terms of wrms repeatability. Together with the results of the re-levellings and the gravity information they are used to separate model ; the anthropogenic and tectonic reasons for the site motions.
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Health Care Policy and Trends Pharmaceutical Environment and Trends Pharmaceutical Market Performance Health Professionals Canadian Drug Distribution Snapshots on Disease and Treatments 3.6.1. ADHD 3.6.2. Hypertension 3.6.3. Parkinson's Disease 3.6.4. Dermatological Preparations 3.6.5. Smoking Cessation 3.6.6. Multiple Sclerosis 3.6.7. Alzheimer's Privacy and Health Research Estrogen Replacement Therapy: Determinants of Persistence with Treatment 27 34 48 and nardil.
Unite Propre de Recherche de l9Enseignement Superieur UPRES ; 2705, Service de Pneumologie, Centre des Maladies Vasculaires Pulmonaires and #UPRES 2704, Service de Pediatrie, Hopital Antoine Beclere, Universite ` Paris Sud, Clamart, zCentre HepatoBiliaire, Hopital Paul Brousse, Villejuif and Service d9Immuno-hematologie ; and Service de Biochimie, Hopital Lariboisiere, Assistance Publique, ` Hopitaux de Paris, Paris, France. Division of Medical Genetics, Depts of Medicine and Genetics, University of Leicester, Leicester, UK. Correspondence: M. Humbert, Service de Pneumologie, Centre des Maladies Vasculaires Pulmonaires, Hopital Antoine Beclere, 157 rue de la Porte ` de Trivaux, 92140 Clamart, France. Fax: 33 146303824 E-mail: humbert ipsc.u-psud Keywords: Pulmonary hypertension, serotonin, type-I glycogen-storage disease Received: July 6 2001 Accepted after revision: January 7 2002 This study was supported by grants from Universite Paris-Sud, Institut National de la Sante et de la Medicale INSERM ; and Association Francaise contre les Myopathies AFM and nadolol.
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